pc3 prostate cancer cells  (ATCC)

Journal: RSC Advances

Article Title: Hybrid benzylidene thiazolidine-2,4-diones as potent apoptosis-inducing anticancer agents: design-driven optimization, cytotoxic profiling, and mechanistic validation in prostate cancer

doi: 10.1039/d6ra01323f

Figure Lengend Snippet: The half-maximal inhibitory concentrations (IC 50 ) for the newly synthesized compounds (HB121–HB169) using PC-3, MCF7, and HCT-116 human cell lines for cancer. Quantitative analysis and graphical representation were performed using GraphPad Prism (version 8.2). Values are expressed as the mean ± standard deviation (SD) of at least three independent experiments. Statistical evaluation was performed using one-way analysis of variance (ANOVA) followed by Tukey's post hoc multiple-comparison test. * P < 0.05 was considered statistically significant compared with doxorubicin.

Article Snippet: All cancer cell lines used in this study (Hepatocellular carcinoma (HuH-7), lung malignancies (A549, H460), breast cancer (MCF-7), osteosarcoma (MG63), colorectal carcinoma (HCT-116), and prostate cancer (PC-3)) were obtained from Vacsera (Giza, Egypt), while the normal human skin fibroblast (HSF) cell line was obtained from the American Type Culture Collection (ATCC, USA).

Techniques: Synthesized, Standard Deviation, Comparison

Journal: RSC Advances

Article Title: Hybrid benzylidene thiazolidine-2,4-diones as potent apoptosis-inducing anticancer agents: design-driven optimization, cytotoxic profiling, and mechanistic validation in prostate cancer

doi: 10.1039/d6ra01323f

Figure Lengend Snippet: (A) Cell cycle studies using flow cytometry of PC-3 cancer cells treated with HB162 in comparison to untreated controls. (B) Representative histograms showing PC-3 cells exposed to HB162 (left) versus control cells (right).

Article Snippet: All cancer cell lines used in this study (Hepatocellular carcinoma (HuH-7), lung malignancies (A549, H460), breast cancer (MCF-7), osteosarcoma (MG63), colorectal carcinoma (HCT-116), and prostate cancer (PC-3)) were obtained from Vacsera (Giza, Egypt), while the normal human skin fibroblast (HSF) cell line was obtained from the American Type Culture Collection (ATCC, USA).

Techniques: Flow Cytometry, Comparison, Control

Journal: Frontiers in Immunology

Article Title: PhIP-driven prostate cancer involves key molecular regulators and immune microenvironment modulation

doi: 10.3389/fimmu.2026.1782240

Figure Lengend Snippet: PhIP exposure induces cytotoxicity in RWPE-1 cells and downregulates SLC14A1 expression in PC-3 cells. (A) Immunohistochemical staining of SLC14A1 in prostate cancer tissues and normal prostate tissues based on data from the HPA database. (B) Cell viability of RWPE-1 cells after treatment with increasing concentrations of PhIP for 48 h, as determined by CCK-8 assay. (C) Protein expression levels of SLC14A1 in PC-3 cells following PhIP treatment, as assessed by Western blot. (D) Relative mRNA expression levels of SLC14A1 in PC-3 cells after PhIP treatment, as determined by qRT-PCR (**p < 0.01, ***p < 0.001).

Article Snippet: The human prostatic epithelial cell line RWPE-1 and the human prostate cancer cell line PC-3 were obtained from the American Type Culture Collection (ATCC).

Techniques: Expressing, Immunohistochemical staining, Staining, CCK-8 Assay, Western Blot, Quantitative RT-PCR